General Cell Based Assays
Cayman Chemical LDH CytotoxIcIty asy KIt 96Wel
For measurement of LDH activity as an indicator of cell death
Cayman Chemical Total nd DIrect BIlIrubIn Co
Cayman’s Total and Direct Bilirubin Colorimetric Assay provides a rapid, reliable, and reproducible colorimetric method for measuring total and direct bilirubin levels in serum. This kit uses a modified Jendrassik-Gróf method, in which total bilirubin concentration is determined by diazo salt in the presence of a catalyst and direct bilirubin is determined in the absence of the catalyst.{55801,55802} The Jendrassik-Gróf method is a widely used clinical method.{55806} However, the results may not be comparable to the results of analytical methods such as mass spectrometry or NMR, and this kit is designed for research use only (not to be used for clinical purposes). The assay has a dynamic range of 0.045-8 mg/dl (0.022-4 μg/well) for both total and direct bilirubin.
Cayman Chemical D-Lactate asy KIt 96Wel
D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles. D-lactate is normally produced in the gastrointestinal tract, mainly by lactobacilli and bifidobacteria or is produced in the cytosol of cells by the glyoxylase pathway. Cayman’s D-Lactate Assay provides a fluorescence-based method for detecting D-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, D-lactate dehydrogenase catalyzes the oxidation of D-lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Cayman Chemical ALT asy InItIatr 1ea
A - and K1-opioid receptor antagonist (Kis 0.3 and 0.2 nM respectively) selective for - and K1-opioid receptors over the -opioid receptor (Ki 12.8 nM) reversibly inhibits electricity-induced twitches of isolated guinea pig ileal longitudinal muscle strips (IC50 48 nM) indicating opioid agonist activity inhibits endomorphin 1-induced conditioned place preference in mice at 0.3 UG/animal i.c.v. prevents decreases in urine output induced by fentanyl D-propoxyphene buprenorphine profadol or bromadoline in water-loaded rats at 40 mg/kg reduces hemokinin 1-induced increases in the latency to withdrawal in the tail-flick test in mice
Cayman Chemical NADH Stndard 1.5mg
A derivative of 6-mercaptopurine has been used as a synthetic intermediate in the synthesis of compounds derived from azathioprine
Cayman Chemical LIpIdLaunchALC0315 LNP KIt 1ea
Cayman’s LipidLaunch™ ALC-0315 LNP Kit (Loadable) provides cargo-ready, empty ALC-0315 LNPs. These LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. LipidLaunch™ LNPs (Loadable) demonstrate very low toxicity compared to traditional lipid-based transfection methods. Conventionally loaded LNPs typically require specialized equipment to ensure consistent control of particle size, while also requiring large reaction volumes. LipidLaunch™ ALC-0315 LNPs (Loadable) enable users to encapsulate cargo in low reaction volumes offering greater flexibility in the scale of preparation while preserving costly nucleic acid cargo.
Cayman Chemical Glycogen asy KIt 96Wel
An ALC-0315-based LNP solution for expressing mCherry composed of ALC-0315 cholesterol DSPC and DMG-PEG(2000) at a molar ratio of 9.442.746.31.6 and encapsulating mRNA encoding mCherry intended for proof-of-concept experiments to determine whether ALC-0315-based LNPs effectively lead to expression of a protein of interest in a target cell type either in vitro or in vivo mCherry has ex/em 587/610 nm respectively
Cayman Chemical Glycogen asy Buffer5X 14mL
A nitroxyl donor reduces MAP and systemic vascular resistance and increases cardiac output in a dog model of microembolism-induced heart failure at four hours post infusion at 7 UG/kg/min for four hours
Cayman Chemical MPA asy Reagent 2g
An activator of the anticipatory unfolded protein response cytotoxic to MCF-7 cells (IC50 42.6 nM) as well as T47D cells at 0.5 and 1 UM
Cayman Chemical PhagocytosIs asy KItIgG F 1ea
Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-FITC Complex for up to 750 samples.
Cayman Chemical PhagocytosIs asy KItIgG-D 1ea
Cayman’s Phagocytosis Assay Kit (IgG-DyLight™ 633) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-DyLight™ 633 Complex for up to 500 samples.
Cayman Chemical Glucose Uptake CelBased As 1ea
Cayman’s Glucose Uptake Cell-Based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. The kit employs NBD-glucose (2-NBDG), a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Researchers interested in cellular metabolism, such as cancer biologists, immunologists, physiologists, and others, will find this kit a robust tool for measuring energy consumption in living cells.
Cayman Chemical AconItase NADPReagent 1ea
A saturated acyl CoA and metabolite of 3-oxovalproyl-CoA hepatic levels of pentanoyl-CoA and propionyl-CoA are increased in an Apc /Min-FCCC mouse model of spontaneous colorectal cancer fed a low- or high-fat diet
Cayman Chemical DMSO asy Reagent 3mL
A DPP-9 inhibitor (IC50 0.0034 UM) selective for DDP-9 over DPP-8 DPP-4 DPP-2 FAP and prolyl endopeptidase (IC50s 0.6 >10 >5 10 and >10 UM respectively) induces LDH release from THP-1 and HL-60 leukemia cells at 10 UM
Cayman Chemical Cel-Based asy TBS10X 10mL
A NUDT15 inhibitor (IC50 25 nM) selective for NUDT15 over a panel of additional Nudix family hydrolases proteins and nucleotide pyrophosphatases at 100 UM as well as a panel of 44 kinases at 10 UM enhances thiopurine-induced cell death in NB4 and HL-60 cancer cells in a NUDT15-dependent manner